Sign in →

Test Code ANAC Antinuclear Antibody Cascade, Serum


Ordering Guidance


This test is performed using a multiplex flow immunoassay method and screens for antibodies to a limited number of nuclear antigens. It does not screen for all antibodies associated with myositis, scleroderma, or autoimmune liver disease. Antinuclear antibody testing methods using HEp-2 substrate are recommended for systemic rheumatic disease screening. For more information see NAIFA / Antinuclear Antibodies, HEp-2 Substrate, IgG, Serum.



Specimen Required


Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Specimen Volume: 0.5 mL


Useful For

Evaluation of patients with signs and symptoms compatible with connective tissue diseases

 

This test is not recommended for:

-Testing in clinical situations with a high prevalence of connective tissue diseases (eg, rheumatology specialty practice)

-Follow-up evaluation of patients with known connective tissue diseases

Profile Information

Test ID Reporting Name Available Separately Always Performed
ANASC Antinuclear Ab Screen, S Yes Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
DSDNA DNA Double-Stranded Ab, IgG, S Yes No
ENAE Ab to Extractable Nuclear Ag Eval,S Yes No
CMA Centromere Ab, IgG, S Yes No
RIB Ribosome P Ab, IgG, S Yes No

Testing Algorithm

If antinuclear antibody screen is positive, then antibodies to double-stranded DNA (dsDNA) and extractable nuclear antigen evaluation are performed at an additional charge.

 

If results from dsDNA antibodies and extractable nuclear antigens are all negative, then centromere and ribosome P antibodies will be performed at an additional charge.

Method Name

Multiplex Flow Immunoassay

Reporting Name

Antinuclear Ab Cascade, S

Specimen Type

Serum

Specimen Minimum Volume

0.4 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 7 days
  Frozen  21 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus OK

Reference Values

Negative

Performing Laboratory

MCHS- La Crosse Lab

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86038

83516 x 2 (if appropriate)

86225 (if appropriate)

86235 x 6 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ANAC Antinuclear Ab Cascade, S 47383-5

 

Result ID Test Result Name Result LOINC Value
ANASC Antinuclear Ab Screen, S 29950-3

Day(s) Performed

Monday through Sunday

Report Available

3 to 4 days