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Test Code DENGS Dengue Virus, Molecular Detection, PCR, Serum


Advisory Information


The presence of dengue virus nucleic acid in serum overlaps with the presence of dengue virus nonstructural protein 1 (NS1) antigen (DNSAG / Dengue Virus NS1 Antigen, Serum). Patients with a history of symptoms for more than 1 week may be negative by molecular tests (ie, real-time PCR) and may require serologic testing (DENVP / Dengue Virus Antibody/Antigen Panel, Serum) to confirm the diagnosis of dengue virus infection.



Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container: Sterile container

Specimen Volume: 0.5 mL

Collection Instructions:

1. Collect whole blood in a serum gel tube.

2. Centrifuge and aliquot the serum into a sterile container within 6 hours of collection.

3. Label specimen as serum.


Useful For

Aiding in the diagnosis of acute infection caused by dengue virus

Testing Algorithm

See Mosquito-borne Disease Laboratory Testing in Special Instructions.

Method Name

Real-Time Polymerase Chain Reaction (PCR)

Reporting Name

Dengue Virus, PCR, Serum

Specimen Type

Serum

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To

Gross hemolysis Reject
Heat-inactivated specimen Reject

Reference Values

Negative

Day(s) and Time(s) Performed

Tuesday, Thursday; 2 p.m.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
DENGS Dengue Virus, PCR, Serum 94427-2

 

Result ID Test Result Name Result LOINC Value
606372 Dengue Virus, PCR, Serum 94427-2