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HelpTest Code PEL Electrophoresis, Protein, Serum
Necessary Information
Indicate if multiple myeloma is suspected.
Specimen Required
Patient Preparation: Fasting (12 hour) preferred but not required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume:1 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial within 2 hours of collection.
Useful For
Monitoring patients with monoclonal gammopathies
Diagnosis of monoclonal gammopathies
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| TPE | Total Protein | Yes, (order TP) | Yes |
| ELP | Protein Electrophoresis | No | Yes |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| IMFX | Immunofixation | No | No |
| IFXED | Immunofixation Delta and Epsilon, S | Yes | No |
Testing Algorithm
If a discrete electrophoresis band is identified, the laboratory will evaluate the serum protein electrophoresis and, if necessary, perform immunofixation at an additional charge.
If a monoclonal light chain is detected in the absence of an associated monoclonal heavy chain, an immunofixation electrophoresis (IFE), specific for delta and epsilon chains, is performed at an additional charge.
If patient history indicates the presence of a previously identified monoclonal heavy chain, specific for delta and epsilon, monoclonal IFE specific for delta and epsilon chains is performed at an additional charge.
Method Name
TPE: Colorimetric, Biuret
ELP: Agarose Gel Electrophoresis
IMFX, IFXED: Immunofixation
Reporting Name
Electrophoresis, Protein, SSpecimen Type
SerumSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 14 days |
| Ambient | 14 days | |
| Frozen | 14 days |
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
PROTEIN, TOTAL
≥1 year: 6.3-7.9 g/dL
Reference values have not been established for patients that are younger than 12 months of age.
PROTEIN ELECTROPHORESIS
Albumin: 3.4-4.7 g/dL
Alpha-1-globulin: 0.1-0.3 g/dL
Alpha-2-globulin: 0.6-1.0 g/dL
Beta-globulin: 0.7-1.2 g/dL
Gamma-globulin: 0.6-1.6 g/dL
An interpretive comment is provided with the report.
Reference values have not been established for patients that are younger than 16 years of age.
Performing Laboratory
MCHS- La Crosse LabTest Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
84155
84165
86334-Immunofixation (if appropriate)
86334-Immunofixation Delta and Epsilon (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| PEL | Electrophoresis, Protein, S | 24351-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| TPE | Total Protein | 2885-2 |
| 2769 | Albumin | 2862-1 |
| 2770 | Alpha-1 Globulin | 2865-4 |
| 2771 | Alpha-2 Globulin | 2868-8 |
| 2773 | Beta-Globulin | 2871-2 |
| 2774 | Gamma-Globulin | 2874-6 |
| 2785 | A/G Ratio | 44429-9 |
| 22308 | M spike | 33358-3 |
| 22309 | M spike | 33358-3 |
| 15254 | Impression | 49296-7 |
Day(s) Performed
Tuesday, Thursday